Fluorescence Discrimination of Pharmacological Effects on the Na<sup>+</sup>–Ca<sup>2+</sup> Exchanger and Sarco-Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase in Mouse Ventricular Cardiomyocytes
نویسندگان
چکیده
We developed a method to evaluate the activity of Na+–Ca2+ exchanger (NCX) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused decrease cytoplasmic Ca2+ concentration, which was inhibited SEA0400, an NCX inhibitor, but not cyclopiazonic acid, SERCA inhibitor. β-Adrenoceptor isoprenaline acid SEA0400. Ellagic phenolic phytochemical, also decreased basal Thus, this using fluorescent specific inhibitors would be useful for evaluation pharmacological agents acting on SERCA.
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ژورنال
عنوان ژورنال: Biological & Pharmaceutical Bulletin
سال: 2021
ISSN: ['1347-5215', '0918-6158']
DOI: https://doi.org/10.1248/bpb.b20-00701